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rabbit anti-vasoactive intestinal peptide (vip) antibody  (Biogenex)

 
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    Biogenex rabbit anti-vasoactive intestinal peptide (vip) antibody
    Rabbit Anti Vasoactive Intestinal Peptide (Vip) Antibody, supplied by Biogenex, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti-vasoactive+intestinal+peptide+(vip)+antibody/rabbit+anti+vasoactive+intestinal+peptide++vip++antibody/pmc03279408-189-20-27
    Average 90 stars, based on 1 article reviews
    rabbit anti-vasoactive intestinal peptide (vip) antibody - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Staining:

    Article Title: Vasoactive intestinal polypeptide immunoreactivity in the human cerebellum: qualitative and quantitative analyses
    Article Snippet: The immunoreactivity was revealed by a rabbit polyclonal anti-VIP antibody (Biogenex). (A) Positive stellate neurons (arrowheads) and basket neurons (arrows). (B) A positive Purkinje neuron displaying diffuse immunoreactivity in the body and apical dendrite cytoplasm; positive neurons of the molecular layer are also seen. (C) Immunoreactivity is observed in the punctate element (putative axon terminals) in the neuropil and on the surface of an immunonegative Purkinje neuron (arrowheads). (D) VIP-positive terminals (arrowheads) in close anatomical relationship with the wall of a microvessel (mv).

    Article Title: Vasoactive intestinal polypeptide immunoreactivity in the human cerebellum: qualitative and quantitative analyses
    Article Snippet: Incubation with primary antibodies The primary antibodies were diluted in a buffer solution (BS) containing 5% fetal calf serum in PBS to the following dilutions: (1) monoclonal mouse anti-VIP (Santa Cruz Biotechnology), 1 : 40; (2) polyclonal rabbit anti-VIP (Biogenex), 1 : 100; (3) polyclonal rabbit anti-VIP (ICN Biomedicals Inc), 1 : 600; and (4) polyclonal goat anti-VIP (Santa Cruz Biotechnology), 1 : 500.

    Article Title: A Non-Mammalian Type Opsin 5 Functions Dually in the Photoreceptive and Non-Photoreceptive Organs of Birds
    Article Snippet: Miles Epstein, University of Wisconsin, USA), rabbit anti-Meis antibody (MAB1614, Millipore), rabbit anti-glutamic acid decarboxylase (GAD) 65/67 antibody (ab11070, Abcam), rabbit anti-vasoactive intestinal peptide (VIP) antibody (AR443-5R, Biogenex), rabbit anti-serotonin antibody (a gift from Dr. David V. Pow, University of Newcastle, Australia), rabbit anti-gonadotropin releasing hormone (GnRH) antibody (ab5617, Abcam), and rabbit anti-vasopressin antibody (T-4563, Bachem).

    Standard Deviation:

    Article Title: Vasoactive intestinal polypeptide immunoreactivity in the human cerebellum: qualitative and quantitative analyses
    Article Snippet: The immunoreactivity was revealed by a rabbit polyclonal anti-VIP antibody (Biogenex). (A) Positive stellate neurons (arrowheads) and basket neurons (arrows). (B) A positive Purkinje neuron displaying diffuse immunoreactivity in the body and apical dendrite cytoplasm; positive neurons of the molecular layer are also seen. (C) Immunoreactivity is observed in the punctate element (putative axon terminals) in the neuropil and on the surface of an immunonegative Purkinje neuron (arrowheads). (D) VIP-positive terminals (arrowheads) in close anatomical relationship with the wall of a microvessel (mv).

    Article Title: Vasoactive intestinal polypeptide immunoreactivity in the human cerebellum: qualitative and quantitative analyses
    Article Snippet: Incubation with primary antibodies The primary antibodies were diluted in a buffer solution (BS) containing 5% fetal calf serum in PBS to the following dilutions: (1) monoclonal mouse anti-VIP (Santa Cruz Biotechnology), 1 : 40; (2) polyclonal rabbit anti-VIP (Biogenex), 1 : 100; (3) polyclonal rabbit anti-VIP (ICN Biomedicals Inc), 1 : 600; and (4) polyclonal goat anti-VIP (Santa Cruz Biotechnology), 1 : 500.

    Article Title: A Non-Mammalian Type Opsin 5 Functions Dually in the Photoreceptive and Non-Photoreceptive Organs of Birds
    Article Snippet: Miles Epstein, University of Wisconsin, USA), rabbit anti-Meis antibody (MAB1614, Millipore), rabbit anti-glutamic acid decarboxylase (GAD) 65/67 antibody (ab11070, Abcam), rabbit anti-vasoactive intestinal peptide (VIP) antibody (AR443-5R, Biogenex), rabbit anti-serotonin antibody (a gift from Dr. David V. Pow, University of Newcastle, Australia), rabbit anti-gonadotropin releasing hormone (GnRH) antibody (ab5617, Abcam), and rabbit anti-vasopressin antibody (T-4563, Bachem).

    Incubation:

    Article Title: Vasoactive intestinal polypeptide immunoreactivity in the human cerebellum: qualitative and quantitative analyses
    Article Snippet: The immunoreactivity was revealed by a rabbit polyclonal anti-VIP antibody (Biogenex). (A) Positive stellate neurons (arrowheads) and basket neurons (arrows). (B) A positive Purkinje neuron displaying diffuse immunoreactivity in the body and apical dendrite cytoplasm; positive neurons of the molecular layer are also seen. (C) Immunoreactivity is observed in the punctate element (putative axon terminals) in the neuropil and on the surface of an immunonegative Purkinje neuron (arrowheads). (D) VIP-positive terminals (arrowheads) in close anatomical relationship with the wall of a microvessel (mv).

    Article Title: Vasoactive intestinal polypeptide immunoreactivity in the human cerebellum: qualitative and quantitative analyses
    Article Snippet: Incubation with primary antibodies The primary antibodies were diluted in a buffer solution (BS) containing 5% fetal calf serum in PBS to the following dilutions: (1) monoclonal mouse anti-VIP (Santa Cruz Biotechnology), 1 : 40; (2) polyclonal rabbit anti-VIP (Biogenex), 1 : 100; (3) polyclonal rabbit anti-VIP (ICN Biomedicals Inc), 1 : 600; and (4) polyclonal goat anti-VIP (Santa Cruz Biotechnology), 1 : 500.

    Article Title: A Non-Mammalian Type Opsin 5 Functions Dually in the Photoreceptive and Non-Photoreceptive Organs of Birds
    Article Snippet: Miles Epstein, University of Wisconsin, USA), rabbit anti-Meis antibody (MAB1614, Millipore), rabbit anti-glutamic acid decarboxylase (GAD) 65/67 antibody (ab11070, Abcam), rabbit anti-vasoactive intestinal peptide (VIP) antibody (AR443-5R, Biogenex), rabbit anti-serotonin antibody (a gift from Dr. David V. Pow, University of Newcastle, Australia), rabbit anti-gonadotropin releasing hormone (GnRH) antibody (ab5617, Abcam), and rabbit anti-vasopressin antibody (T-4563, Bachem).



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    Vasoactive intestinal peptide receptors in mice colon during DSS colitis with sleep fragmentation. ( A ) Immunohistochemical (IHC) staining of <t>VPAC1</t> and VPAC2 in colon sections. Upper part: expression of VPAC1 in colon tissue of different groups at 200× & 400× magnification. Comparison of the VPAC1 in five groups was shown in ( B ) right upper panel. ( A ) Lower part: expression of VPAC2 in colon tissue of different groups at 200× & 400× magnification. Comparison of the VPAC2 in five groups was shown in ( B ) right middle panel. Comparison of VPAC1/VPAC2 ratios by sleep fragmentation in normal and inflamed state was shown in ( B ) right lower panel. Quantification of area percentage of IHC staining by true color image analysis with the application of adjusted thresholds. ( C ) Western blot analysis of VPAC2 and β-actin (loading control) in colon homogenates. Right graph indicates quantification relative to β-actin. * Stands for a result of five groups comparison (represented by bold line segments with endpoints) The uncropped western blot figures were presented in . The densitometry readings/intensity ratio of VPAC2 in western blots in DSS-colitis mice with sleep fragmentation were presented in . ✠, ✟, #, $, & and € represent the results of control group versus DSS group, DSS group versus DSS + SF group, DSS + SF group versus DSS + SF + EA group, control group versus DSS + SF group, control group versus DSS + SF + EA group, and control group versus SF group, respectively (represented as a thin line without endpoints).*, ✠, ✟, #, $, &, €, p < 0.05; **, ✠✠, ✟✟, ##, $$, &&, €€ p < 0.01; ***, p < 0.001; ns, no significance. Data were presented as mean ± SEM of repeated adjusted thresholds in each group. VPAC1, vasoactive intestinal peptide (VIP) <t>type</t> <t>1</t> receptor; VPAC2, vasoactive intestinal peptide (VIP) type 2 receptor; DSS, dextran sodium sulfate; SF, sleep fragmentation; EA, electroacupuncture.
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    Vasoactive intestinal peptide receptors in mice colon during DSS colitis with sleep fragmentation. ( A ) Immunohistochemical (IHC) staining of <t>VPAC1</t> and VPAC2 in colon sections. Upper part: expression of VPAC1 in colon tissue of different groups at 200× & 400× magnification. Comparison of the VPAC1 in five groups was shown in ( B ) right upper panel. ( A ) Lower part: expression of VPAC2 in colon tissue of different groups at 200× & 400× magnification. Comparison of the VPAC2 in five groups was shown in ( B ) right middle panel. Comparison of VPAC1/VPAC2 ratios by sleep fragmentation in normal and inflamed state was shown in ( B ) right lower panel. Quantification of area percentage of IHC staining by true color image analysis with the application of adjusted thresholds. ( C ) Western blot analysis of VPAC2 and β-actin (loading control) in colon homogenates. Right graph indicates quantification relative to β-actin. * Stands for a result of five groups comparison (represented by bold line segments with endpoints) The uncropped western blot figures were presented in . The densitometry readings/intensity ratio of VPAC2 in western blots in DSS-colitis mice with sleep fragmentation were presented in . ✠, ✟, #, $, & and € represent the results of control group versus DSS group, DSS group versus DSS + SF group, DSS + SF group versus DSS + SF + EA group, control group versus DSS + SF group, control group versus DSS + SF + EA group, and control group versus SF group, respectively (represented as a thin line without endpoints).*, ✠, ✟, #, $, &, €, p < 0.05; **, ✠✠, ✟✟, ##, $$, &&, €€ p < 0.01; ***, p < 0.001; ns, no significance. Data were presented as mean ± SEM of repeated adjusted thresholds in each group. VPAC1, vasoactive intestinal peptide (VIP) <t>type</t> <t>1</t> receptor; VPAC2, vasoactive intestinal peptide (VIP) type 2 receptor; DSS, dextran sodium sulfate; SF, sleep fragmentation; EA, electroacupuncture.
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    Vasoactive intestinal peptide receptors in mice colon during DSS colitis with sleep fragmentation. ( A ) Immunohistochemical (IHC) staining of <t>VPAC1</t> and VPAC2 in colon sections. Upper part: expression of VPAC1 in colon tissue of different groups at 200× & 400× magnification. Comparison of the VPAC1 in five groups was shown in ( B ) right upper panel. ( A ) Lower part: expression of VPAC2 in colon tissue of different groups at 200× & 400× magnification. Comparison of the VPAC2 in five groups was shown in ( B ) right middle panel. Comparison of VPAC1/VPAC2 ratios by sleep fragmentation in normal and inflamed state was shown in ( B ) right lower panel. Quantification of area percentage of IHC staining by true color image analysis with the application of adjusted thresholds. ( C ) Western blot analysis of VPAC2 and β-actin (loading control) in colon homogenates. Right graph indicates quantification relative to β-actin. * Stands for a result of five groups comparison (represented by bold line segments with endpoints) The uncropped western blot figures were presented in . The densitometry readings/intensity ratio of VPAC2 in western blots in DSS-colitis mice with sleep fragmentation were presented in . ✠, ✟, #, $, & and € represent the results of control group versus DSS group, DSS group versus DSS + SF group, DSS + SF group versus DSS + SF + EA group, control group versus DSS + SF group, control group versus DSS + SF + EA group, and control group versus SF group, respectively (represented as a thin line without endpoints).*, ✠, ✟, #, $, &, €, p < 0.05; **, ✠✠, ✟✟, ##, $$, &&, €€ p < 0.01; ***, p < 0.001; ns, no significance. Data were presented as mean ± SEM of repeated adjusted thresholds in each group. VPAC1, vasoactive intestinal peptide (VIP) <t>type</t> <t>1</t> receptor; VPAC2, vasoactive intestinal peptide (VIP) type 2 receptor; DSS, dextran sodium sulfate; SF, sleep fragmentation; EA, electroacupuncture.
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    Image Search Results


    Vasoactive intestinal peptide receptors in mice colon during DSS colitis with sleep fragmentation. ( A ) Immunohistochemical (IHC) staining of VPAC1 and VPAC2 in colon sections. Upper part: expression of VPAC1 in colon tissue of different groups at 200× & 400× magnification. Comparison of the VPAC1 in five groups was shown in ( B ) right upper panel. ( A ) Lower part: expression of VPAC2 in colon tissue of different groups at 200× & 400× magnification. Comparison of the VPAC2 in five groups was shown in ( B ) right middle panel. Comparison of VPAC1/VPAC2 ratios by sleep fragmentation in normal and inflamed state was shown in ( B ) right lower panel. Quantification of area percentage of IHC staining by true color image analysis with the application of adjusted thresholds. ( C ) Western blot analysis of VPAC2 and β-actin (loading control) in colon homogenates. Right graph indicates quantification relative to β-actin. * Stands for a result of five groups comparison (represented by bold line segments with endpoints) The uncropped western blot figures were presented in . The densitometry readings/intensity ratio of VPAC2 in western blots in DSS-colitis mice with sleep fragmentation were presented in . ✠, ✟, #, $, & and € represent the results of control group versus DSS group, DSS group versus DSS + SF group, DSS + SF group versus DSS + SF + EA group, control group versus DSS + SF group, control group versus DSS + SF + EA group, and control group versus SF group, respectively (represented as a thin line without endpoints).*, ✠, ✟, #, $, &, €, p < 0.05; **, ✠✠, ✟✟, ##, $$, &&, €€ p < 0.01; ***, p < 0.001; ns, no significance. Data were presented as mean ± SEM of repeated adjusted thresholds in each group. VPAC1, vasoactive intestinal peptide (VIP) type 1 receptor; VPAC2, vasoactive intestinal peptide (VIP) type 2 receptor; DSS, dextran sodium sulfate; SF, sleep fragmentation; EA, electroacupuncture.

    Journal: Biology

    Article Title: Alterations in Gut Microbiota and Upregulations of VPAC2 and Intestinal Tight Junctions Correlate with Anti-Inflammatory Effects of Electroacupuncture in Colitis Mice with Sleep Fragmentation

    doi: 10.3390/biology11070962

    Figure Lengend Snippet: Vasoactive intestinal peptide receptors in mice colon during DSS colitis with sleep fragmentation. ( A ) Immunohistochemical (IHC) staining of VPAC1 and VPAC2 in colon sections. Upper part: expression of VPAC1 in colon tissue of different groups at 200× & 400× magnification. Comparison of the VPAC1 in five groups was shown in ( B ) right upper panel. ( A ) Lower part: expression of VPAC2 in colon tissue of different groups at 200× & 400× magnification. Comparison of the VPAC2 in five groups was shown in ( B ) right middle panel. Comparison of VPAC1/VPAC2 ratios by sleep fragmentation in normal and inflamed state was shown in ( B ) right lower panel. Quantification of area percentage of IHC staining by true color image analysis with the application of adjusted thresholds. ( C ) Western blot analysis of VPAC2 and β-actin (loading control) in colon homogenates. Right graph indicates quantification relative to β-actin. * Stands for a result of five groups comparison (represented by bold line segments with endpoints) The uncropped western blot figures were presented in . The densitometry readings/intensity ratio of VPAC2 in western blots in DSS-colitis mice with sleep fragmentation were presented in . ✠, ✟, #, $, & and € represent the results of control group versus DSS group, DSS group versus DSS + SF group, DSS + SF group versus DSS + SF + EA group, control group versus DSS + SF group, control group versus DSS + SF + EA group, and control group versus SF group, respectively (represented as a thin line without endpoints).*, ✠, ✟, #, $, &, €, p < 0.05; **, ✠✠, ✟✟, ##, $$, &&, €€ p < 0.01; ***, p < 0.001; ns, no significance. Data were presented as mean ± SEM of repeated adjusted thresholds in each group. VPAC1, vasoactive intestinal peptide (VIP) type 1 receptor; VPAC2, vasoactive intestinal peptide (VIP) type 2 receptor; DSS, dextran sodium sulfate; SF, sleep fragmentation; EA, electroacupuncture.

    Article Snippet: In addition, the role of the vasoactive intestinal peptide (VIP) type 1 receptor (VIPR1/VPAC1, CSB-PA052529, Cusabio, Houston, TX, USA) and type 2 receptor (VIPR2/VPAC2, A03768, Boster, Pleasanton, CA, USA) under EA were clarified by immunohistochemical staining.

    Techniques: Immunohistochemical staining, Immunohistochemistry, Expressing, Western Blot